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KMID : 0364820120480040240
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.240 ~ p.245
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Genotypic Investigation of Multidrug-Resistant Pseudomonas aeruginosa from Clinical Isolates in Korea, 2010
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Kim Min-Ji
Cha Min-Kyeong
Lee Do-Kyung
Kang Joo-Yeon
Park Jae-Eun
Kim Young-Hee
Park Il-Ho
Shin Hea-Soon
Ha Nam-Joo
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Abstract
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Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that causes serious infection, particularly in
immunocompromised patients. Also, P. aeruginosa possessing carbapenem-resistant metallo-β-lactamases (MBL)
has been reported with increasing frequency in Korea. We therefore analyzed the level of multidrug-resistant clinical
P. aeruginosa isolated from a secondary hospital in Korea in 2010. A total of 92 isolates of P. aeruginosa were
collected from Sahmyook Medical Center in 2010. Susceptibility to antimicrobial agents was determined by analysis
of the minimum inhibitory concentration test; the inhibitor-potentiated disk diffusion (IPD) test was performed for
MBL detection. RAPD-PCR was used for genotyping to rapidly characterize P. aeruginosa strains isolated from
clinical patients. The percentages of non-susceptible isolates were as follows: 40.2% to ceftazidime, 58.7% to
meropenem, 56.5% to gentamicin, 46.7% to tobramycin, 62.0% to ciprofloxacin and 97.8% to chloramphenicol. The
29 multidrug-resistant strains were screened by the IPD test: of the 21 PCR-positive isolates, 19 were IPM-1
producers and 2 were VIM-2 producers. Among the 19 IMP-1-producing P. aeruginosa isolates, 16 isolates showed
similar patterns, and three different banding patterns were observed. The proportion of IMP-1-producing
multidrug-resistant P. aeruginosa from clinical isolates steadily increased in this secondary hospital in Korea in
2010. This study provides information about the antimicrobial-resistant patterns and genotype of multidrug-resistant
P. aeruginosa isolated from clinical isolates in Korea, 2010.
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KEYWORD
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Pseudomonas aeruginosa, antimicrobial resistance, metallo-β-lactamases (MBL), multidrug-resistant
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